cTnI from the sample becomes labeled with the ALP-conjugated antibody and is also captured on to the sensor floor through the incubation phase.[49] Wash fluid that contains enzyme substrate is then placed on remove unbound substances, and at the same time, ALP sure to the antigen-antibody advanced reacts, releasing an electrochemically detectable solution. The created amperometric signal is immediately proportional into the cTnI inside the sample.[fifty]

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